cd182 cxcr2 apc  (Miltenyi Biotec)

Journal: BMC Medical Genomics

Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

doi: 10.1186/s12920-026-02335-4

Figure Lengend Snippet: Identification of the key genes expression in external datasets. A IFIT5, IFI16, TRIM22, IFI44L, IRF9, OAS3, BST2, CASP1, CXCR2 were statistically significant in AF expression profile ( GSE41177 ). B IFIT5、CASP1 and CXCR2 were statistically significant in COPD expression profile ( GSE56766 ). C - D ROC of IFIT5、CASP1 and CXCR2 in the AF( GSE79768 ) dataset. -, not significant.;*, p <0.05; **, p <0.01; ***, p < 0.001

Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

Techniques: Expressing

Journal: BMC Medical Genomics

Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

doi: 10.1186/s12920-026-02335-4

Figure Lengend Snippet: Validation of CASP1, CXCR2, and IFIT5 expression in different clinical groups at mRNA and protein levels. A – C Relative mRNA expression levels of CASP1, IFIT5, and CXCR2 were assessed by qRT-PCR in peripheral blood samples from healthy controls (CON), atrial fibrillation (AF), chronic obstructive pulmonary disease (COPD), and AF combined with COPD (AF+COPD). D – F Quantification of CASP1, IFIT5, and CXCR2 protein expression levels based on Western blot analysis. Signal intensities were normalized to GAPDH. G – I Serum protein concentrations of CASP1 (pmol/L), IFIT5 (pg/mL), and CXCR2 (ng/mL) measured by ELISA in the four groups. J Representative Western blot images for CASP1, IFIT5, and CXCR2 with GAPDH as loading control. *, p < 0.05; **, p <0.01; ***, p < 0.001

Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: BMC Medical Genomics

Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

doi: 10.1186/s12920-026-02335-4

Figure Lengend Snippet: A Transcription factor (TF)-gene interaction network based on key genes. B miRNA-gene interaction network based on key genes. C , E , G Overall docking models of CASP1, CXCR2, and IFIT5 proteins with estradiol, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes. D , F , H Overall docking models of CASP1, CXCR2, and IFIT5 proteins with methyl methanesulfonate, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes

Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

Techniques: